|Title||Endophenotype Growth Response Of Allium Cepa L. Under Salinity Stress
Ambit Journal of Biotechnology (Ajb). September Vol. 1(2)
|Authors||DURU, C. M., OSIKWE, A. K. and OKECHUKWU, R. I.|
|Published on||19 September 2015 Pages: 09-15 Article Id : AJB003|
|Abstract | PDF | Cited By
This study investigated the Endophenotype growth kinetics of Allium cepa L., in response to variations in the concentration of aqueous sodium chloride (NaCl) solution; using cytogenetic approach. The aim was to establish an alternative parametric index (%proportion of mitotic cells per field), as well as to ascertain the possibility of cultivating A. cepa L in our saliferous coastal belt. Dried, shrivelled roots of A. cepa bulb were shorn off and the stem surface dipped in a beaker of distilled water, 0.00mMNaCl (control) and various concentrations, (10- 500mMNaCl) sodium chloride solution. Fresh roots were harvested after 72 hours, fixed for 24 hours in Carnoy’s fluid, macerated and squashed on slides. The number of dividing cells per field, per concentration was counted. The results, as depicted in the growth trajectory, showed that, the relative proportion of the dividing cells per field, increased with increase in sodium chloride concentrations; from control (12%) till 250mM NaCl (32%) and dropped pendulously to 350mM NaCl (13%). The concentrations of 400mM to 500mM did not support root emergence. The relevance of this approach to the discipline of plant Physiology, Cytogenetics, Ecology and Agriculture was implied.