Table of Content, July 2015 | AJB

Title Antioxidant - Mediated Glutathione Levels Of Glucose- 6 - Phosphate Dehydrogenase - Deficient Erythrocytes Under Drug-induced Oxidative Stress
Ambit Journal of Biotechnology (Ajb). July Vol. 1(1)
Authors ALOH, G. S and Obeagu,E.I., Okoroiwu,I.L., Odo,C.E., Kanu, S.N., Elemchukwu, Q., Okpara, K.E. and Ugwu, G.U
Published on 28 July 2015     Pages: 04-08     Article Id : AJB002    
Abstract | PDFCited By

The effects of two antioxidants, ascorbic acid and α– tocopherol, on the glutathione (GSH) levels of glucose – 6 – phosphate dehydrogenase (G6PD) – deficient erythrocytes were studied before inducing oxidative stress with acetylphenylhydrazine (APHZ), and after inducing the stress, followed separately by ascorbic acid and α – tocopherol treatments. Before APHZ treatment, the mean GSH levels in the control and G6PD – deficient erythrocytes were 38.82 + 1.48 mg/100ml and 36.00 + 2.44mg/100ml respectively. Their respective mean post – APHZ levels were 37.00 + 2.35 mg/100ml and 17.00+ 0.01mg/100ml. Post – APHZ + ascorbic acid, the control’s mean GSH level was 37.75 + 2.50mg/100ml and the mean level in the G6PD – deficient cells were 27.00+ 3.44mg/100ml, while their respective post – APHZ + α– tocopherol levels were 38.76 + 2.00mg/100ml (control) and 33.00 + 1.93mg/100ml (G6PD – deficient red cells). The results of this study indicate that the initial effect of an oxidant drug on erythrocytes is to lower their GSH levels. This effect was found to be more severe in G6PD – deficient red cells than in the non – deficient ones. Ascorbic acid and α– tocopherol could ameliorate the effect of this oxidant stressor, and possibly others, but the latter was more effective. Its fat – solubility is suspected to be responsible for this enhanced efficacy.

Title Antioxidant- Mediated Glutathione Levels Of Sickle Erythrocytes Under Drug - Induced Oxidative Stress
Ambit Journal of Biotechnology (Ajb). July Vol. 1(1)
Authors ALOH, G. S and Obeagu,E.I., Okoroiwu,I.L., Odo,C.E., Kanu, S.N., Elemchukwu, Q., Okpara, K.E. and Ugwu, G.U
Published on 28 July 2015     Pages: 01-14     Article Id : AJB001    
Abstract | PDFCited By

The effects of two antioxidants, ascorbic acid and a-tocopherol, on the glutathione (GSH) levels of sickle erythrocytes were studied before and after inducing oxidative stress with 5mg of acetylphenylhydrazine (APHZ) per ml of blood. Pre –APHZ GSH levels of the control and sickle erythrocytes were 39.82 + 1.48 mg/100ml and 26.00 + 2.32 mg/ml respectively. Their respective post – APHZ levels were 37.00+ 2.35 mg/ml and 13.00 + 1.98 mg /ml. Post – APHZ +ascorbic acid, their mean GSH levels were respectively 37.75 + 2.50 mg/ml and 16.00 + 1.83 mg/ml , while post- APHZ + a- tocopherol significantly (p<0.05) elevated their GSH levels to 39.76 + 2.80 mg/ml and 22.00 + 2.24 mg/ml respectively. These results indicate that whether defective or not, the initial effect of an oxidant drug is to lower the GSH levels of red cells. Because the sickle erythrocytes had a subsisting redox stress, the effect of APHZ could not be fully reversed by both ascorbic acid and a - tocopherol as occurred in the control. a – Tocopherol was more effective in counteracting the effect of APHZ, probably because it is a fat – soluble vitamin.